Calligraphy appreciation is widely considered to require sequence restoration, while the sequence restoration of painting is ignored in long time. Moreover, the detail feature of brush strokes is considered to enhance the perception of sequentiality. In order to quantify the sequentiality perception and to explore the influence of detail features of strokes on sequentiality perception, a sequentiality perception quantization method based on Markov chain entropy rate was proposed. Firstly, the perceived sequentiality of an individual to the markers on the artwork was modeled as a Markov chain. Then, the entropy rate of the Markov model was calculated to measure the uncertainty of the perceived sequentiality. Finally, the negentropy was used to measure the order of the perceived sequentiality and was normalized to obtain the measurement index:sequentiality perception. The feasibility of this method was verified through the actual measurement of the sequentiality perception of a group of artworks. And based on the proposed sequentiality measurement, the effect of graph transformation on the sequentiality perception of artworks was studied. Experimental results show that the sequentiality consistency keeps high level when rotation or mirror transformation is performed to the original image, while the correctness varies significantly. This means that the feature of brush strokes is not the primary factor in forming the sequentiality experience, and the viewer's own experience of order of strokes is more important in the formation, but this conclusion needs to be further verified.